An egg-yolk-free extender for refrigerated cat sperm
Semen extenders and cryopreservation
Gaeth et al. · Theriogenology · 10.1016/j.theriogenology.2026.118279
A defined synthetic extender kept refrigerated domestic cat epididymal sperm motile longer than the Tris and egg-yolk comparator in laboratory tests. The authors did not test fertilizing capacity after extended storage.
Altmanová et al. · Veterinary Medicine and Science · Case report
A study of a captive breeding pair identified semen quality as a likely factor in infertility. The authors combined reproductive and cytogenetic assessments.
None of 38 archived serum samples in the selected progesterone range returned a positive rapid LH result. The study cautions against using that range alone to identify the LH surge and did not establish a new threshold.
Egg yolk extender and membrane lipids in chilled dog semen
Semen extenders and cryopreservation
Temerario et al. · Animals · 10.3390/ani16152311
In laboratory storage tests, a Tris and egg yolk extender slowed the loss of selected sperm membrane lipids and helped preserve early motility. The researchers did not measure pregnancy outcomes.
Heidari et al. · BMC Veterinary Research · 10.1186/s12917-026-05538-0
Adding apigenin and N-acetylcysteine amide to a Tris and egg yolk extender improved laboratory measures during chilled storage of pooled semen from five dogs. The experiment did not test fertility.
Endoscopic insemination with frozen epididymal dog sperm
Artificial insemination and clinical techniques
Bacsa et al. · Veterinary Sciences · Case report · 10.3390/vetsci13040326
A dog delivered nine puppies after endoscope-assisted transcervical insemination using frozen-thawed epididymal sperm. This single case shows feasibility under the reported conditions and cannot establish a success rate.
Pulkowska-Bluj et al. · Polish Journal of Veterinary Sciences · 10.24425/pjvs.2026.158499
In samples from ten dogs, the Tris extender with egg yolk preserved selected post-thaw motility and mitochondrial measures better than the tested soy lecithin formulations. Fertility was not measured.
Vansandt et al. · Reproduction and Fertility · 10.1530/RAF-25-0074
A study of ovarian synchronization and laparoscopic insemination in jaguars reported a live cub. The final experiment involved five females, so the result does not establish a reliable pregnancy rate.
Reeves et al. · Theriogenology Wild · 10.1016/j.therwi.2026.100146
A field study of six ocelots and six bobcats found no detectable differences between ultra-rapid and conventional straw freezing on the laboratory outcomes tested. Fertilization tests used domestic cat oocytes and did not assess births.
Sumayya et al. · The Indian Journal of Animal Sciences · 10.56093/ijans.v94i12.153123
In chilled semen samples from 15 dogs, the tested Tris extender with egg yolk performed better than the soy lecithin formulation on several laboratory measures. DNA integrity did not differ and pregnancy was not assessed.