FelidsAI

Veterinary Reproduction Library

Research Blog

Research on felid and canine reproduction, from semen extenders and insemination to embryology and conservation.

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Research notes

An egg-yolk-free extender for refrigerated cat sperm

Semen extenders and cryopreservation

Gaeth et al. · Theriogenology · 10.1016/j.theriogenology.2026.118279

A defined synthetic extender kept refrigerated domestic cat epididymal sperm motile longer than the Tris and egg-yolk comparator in laboratory tests. The authors did not test fertilizing capacity after extended storage.

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Infertility in a captive jaguar

Altmanová et al. · Veterinary Medicine and Science · Case report

A study of a captive breeding pair identified semen quality as a likely factor in infertility. The authors combined reproductive and cytogenetic assessments.

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Reproductive technologies and biobanking for wild felids

Deco-Souza et al. · Theriogenology

This review examines how assisted reproduction and the preservation of reproductive material can support wild felid conservation.

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Canine progesterone and timing of the LH surge

Hormones, cycles and timing

Petré et al. · Theriogenology · 10.1016/j.theriogenology.2026.118214

None of 38 archived serum samples in the selected progesterone range returned a positive rapid LH result. The study cautions against using that range alone to identify the LH surge and did not establish a new threshold.

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Reproductive measurements in Caatinga oncillas

Matos et al. · Zoo Biology

The study describes testicular ultrasound measurements and semen characteristics in captive oncillas from the Caatinga biome.

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Egg yolk extender and membrane lipids in chilled dog semen

Semen extenders and cryopreservation

Temerario et al. · Animals · 10.3390/ani16152311

In laboratory storage tests, a Tris and egg yolk extender slowed the loss of selected sperm membrane lipids and helped preserve early motility. The researchers did not measure pregnancy outcomes.

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Antioxidants in an extender for chilled dog semen

Semen extenders and cryopreservation

Heidari et al. · BMC Veterinary Research · 10.1186/s12917-026-05538-0

Adding apigenin and N-acetylcysteine amide to a Tris and egg yolk extender improved laboratory measures during chilled storage of pooled semen from five dogs. The experiment did not test fertility.

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Endoscopic insemination with frozen epididymal dog sperm

Artificial insemination and clinical techniques

Bacsa et al. · Veterinary Sciences · Case report · 10.3390/vetsci13040326

A dog delivered nine puppies after endoscope-assisted transcervical insemination using frozen-thawed epididymal sperm. This single case shows feasibility under the reported conditions and cannot establish a success rate.

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Soy lecithin and egg yolk in frozen dog semen

Semen extenders and cryopreservation

Pulkowska-Bluj et al. · Polish Journal of Veterinary Sciences · 10.24425/pjvs.2026.158499

In samples from ten dogs, the Tris extender with egg yolk preserved selected post-thaw motility and mitochondrial measures better than the tested soy lecithin formulations. Fertility was not measured.

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A jaguar cub born after laparoscopic insemination

Artificial insemination and clinical techniques

Vansandt et al. · Reproduction and Fertility · 10.1530/RAF-25-0074

A study of ovarian synchronization and laparoscopic insemination in jaguars reported a live cub. The final experiment involved five females, so the result does not establish a reliable pregnancy rate.

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Field semen banking in wild ocelots and bobcats

Semen extenders and cryopreservation

Reeves et al. · Theriogenology Wild · 10.1016/j.therwi.2026.100146

A field study of six ocelots and six bobcats found no detectable differences between ultra-rapid and conventional straw freezing on the laboratory outcomes tested. Fertilization tests used domestic cat oocytes and did not assess births.

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Soy lecithin and egg yolk in chilled dog semen

Semen extenders and cryopreservation

Sumayya et al. · The Indian Journal of Animal Sciences · 10.56093/ijans.v94i12.153123

In chilled semen samples from 15 dogs, the tested Tris extender with egg yolk performed better than the soy lecithin formulation on several laboratory measures. DNA integrity did not differ and pregnancy was not assessed.

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